Thalassiosira weissflogii  (Grun.) Fryxell et Hasle

New daughter set created every three weeks

Daughter set……….…23umol quanta m-2 s-1

Mother set………….….40umol quanta m-2 s-1

Grandmother set….….16umol quanta m-2 s-1

Q+A:

Q: I have been maintaining CCMP1336 in small cultures in marine minimal media and L1 with a light/dark cycle of 8/16 with approx 30-50 umol m^-2 s^-1 of light and at ~18 °C. My cells seem to be losing their color and are mostly opaque now. When they arrived, they were mostly green. Do you have ideas how to get their pigment back.

A: We maintain cultures with a light/dark regime of 13/11 at ~100uM light. With the regime you are running the cultures may not be getting enough light.

Q: Do you agitate the cultures at all?

A: We do not agitate cultures unless we reach volumes greater than 20L, and then they are agitated with air through an inline filter.

Documentation:

Light backscattering properties of marine phytoplankton: relationships to cell size, chemical composition and taxonomy (https://academic.oup.com/plankt/article/26/2/191/1490132)

The elemental composition of some marine phytoplankton (https://onlinelibrary.wiley.com/doi/abs/10.1111/j.0022-3646.2003.03-090.x)

Photosynthetic architecture differs in coastal and oceanic diatoms (https://www.nature.com/articles/nature02954)

Uptake and subcellular distribution of aluminum in a marine diatom (https://www.sciencedirect.com/science/article/abs/pii/S014765131831100X)

Growth of postlarval sea scallops, Placopecten magellanicus, on microalgal diets, with emphasis on the nutritional role of lipids and fatty acids https://www.sciencedirect.com/science/article/abs/pii/S0044848603007476)

Comparison of early life history stages of the bay scallop, Argopecten irradians: Effects of microalgal diets on growth and biochemical composition (https://www.sciencedirect.com/science/article/abs/pii/S004484860600442X)

Virus infection of Emiliania huxleyi deters grazing by the copepod Acartia tonsa (https://academic.oup.com/plankt/article/38/5/1194/2452783)

Physiological adjustments and transcriptome reprogramming are involved in the acclimation to salinity gradients in diatoms (https://pubmed.ncbi.nlm.nih.gov/27236063/)

Potential of lipid metabolism in marine diatoms for biofuel production (https://pubmed.ncbi.nlm.nih.gov/25763104/)

Preferential utilization of inorganic polyphosphate over other bioavailable phosphorus sources by the model diatoms Thalassiosira spp. (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6849833/)

A revised assessment of the most probable number (MPN) method for enumerating viable phytoplankton cells in ballast water discharge (https://www.researchgate.net/publication/301690007_A_Revised_Assessment_of_the_Most_Probable_Number_MPN_Method_for_Enumerating_Viable_Phytoplankton_Cells_in_Ballast_Water_Discharge)

Ectoine from bacterial and algal origin is a compatible solute in microalgae (https://www.mdpi.com/1660-3397/18/1/42)

Allelopathic inhibition of competing phytoplankton by North American strains of the toxic dinoflagellate, Alexandrium fundyense: Evidence from field experiments, laboratory experiments, and bloom events (https://www.sciencedirect.com/science/article/abs/pii/S1568988311000904)

Effects of episodic turbulence on diatom mortality and physiology, with a protocol for the use of Evans Blue stain for live–dead determinations (https://link.springer.com/article/10.1007/s10750-014-1927-0)

Efficiency of the CO2-concentrating mechanism of diatoms (https://pubmed.ncbi.nlm.nih.gov/21321195/)

Best practices in the flow cytometry of microalgae (https://onlinelibrary.wiley.com/doi/full/10.1002/cyto.a.24328)

Remineralization of bioavailable iron by a heterotrophic dinoflagellate (https://www.researchgate.net/publication/230561452_Remineralization_of_bioavailable_iron_by_a_heterotrophic_dinoflagellate)

A bacterium that inhibits the growth of Pfiesteria piscicida and other dinoflagellates (https://www.sciencedirect.com/science/article/abs/pii/S1568988304000253)

Effects of the pH/pCO2 control method on medium chemistry and phytoplankton growth (https://bg.copernicus.org/articles/6/1199/2009/)

Ammonium uptake and growth limitation in marine phytoplankton (https://aslopubs.onlinelibrary.wiley.com/doi/abs/10.4319/lo.2007.52.6.2496)

Influence of low light and a light:dark cycle on NO3- uptake, intracellular NO3- and nitrogen isotope fractionation by marine phytoplankton (https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1529-8817.2004.03171.x)

Chitin in diatoms and its association with the cell wall (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2708456/)

Irradiance and the elemental stoichiometry of marine phytoplankton (https://aslopubs.onlinelibrary.wiley.com/doi/10.4319/lo.2006.51.6.2690)

Growth inhibition and toxicity of the diatom aldehyde 2-trans, 4-trans-decadienal on Thalassiosira weissflogii (Bacillariophyceae) (https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1529-8817.2005.04052.x)

Supplementation of a diatom diet with cholesterol can enhance copepod egg-production rates (https://aslopubs.onlinelibrary.wiley.com/doi/abs/10.4319/lo.2004.49.2.0488)

Nickel limitation and zinc toxicity in a urea-grown diatom (https://aslopubs.onlinelibrary.wiley.com/doi/abs/10.4319/lo.2008.53.6.2462)

The effects of Cu and Fe availability on the growth and Cu:C ratios of marine diatoms (https://aslopubs.onlinelibrary.wiley.com/doi/abs/10.4319/lo.2008.53.6.2451)

Extracellular production of superoxide by marine diatoms: contrasting effects on iron redox chemistry and bioavailability (https://aslopubs.onlinelibrary.wiley.com/doi/abs/10.4319/lo.2005.50.4.1172)

Assay optimization and regulation of urease activity in two marine diatoms (https://pubmed.ncbi.nlm.nih.gov/29544013/)

A role for manganese in superoxide dismutases and growth of iron deficient diatoms (https://aslopubs.onlinelibrary.wiley.com/doi/abs/10.4319/lo.2004.49.5.1774)

Copper requirements for iron acquisition and growth of coastal ad oceanic diatoms (https://aslopubs.onlinelibrary.wiley.com/doi/abs/10.4319/lo.2005.50.4.1149)

Lipid and fatty acid composition of diatoms revisited: rapid wound-activated change of food quality parameters influences herbivorous copepod reproductive success (https://pubmed.ncbi.nlm.nih.gov/17541989/)

A stress surveillance system based on calcium and nitric oxide in marine diatoms (https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.0040060)

Glutathione and other low molecular weight thiols in marine phytoplankton under metal stress (https://www.int-res.com/abstracts/meps/v232/p93-103/)

Variation in diatom biochemical composition during a simulated bloom and its effect on copepod production (https://academic.oup.com/plankt/article/31/11/1391/1442917)

Phylogenetic diversity in cadmium:phosphorus ratio regulation by marine phytoplankton (https://aslopubs.onlinelibrary.wiley.com/doi/abs/10.4319/lo.2007.52.3.1131)

Characterization of sulfate assimilation in marine algae focusing on the enzyme 5'-Adenylylsulfate reductase (https://academic.oup.com/plphys/article/123/3/1087/6087620)