Thalassiosira weissflogii

Q:       I was wondering whether you could share information about CCMP1336 their growth rates (in L1 medium). We are using it for a time-sensitive project and I would like to have a better understanding of which species had high growth rates. 

A:       CCMP333 is a fairly slow grower (3wks at 14C) so maybe 6-8 weeks to ship 1L.  CCMP1336 and 1320 are aqua culture so could ship 1L in 2-3 weeks.  The remainder are cryo preserved and will take 2-4 months to ship 1L.

Q:       I have been maintaining CCMP1336 in small cultures in marine minimal media and L1 with a light/dark cycle of 8/16 with approx 30-50 umol m^-2 s^-1 of light and at ~18 °C. My cells seem to be losing their color and are mostly opaque now. When they arrived, they were mostly green. Do you have ideas how to get their pigment back.

A:       We maintain cultures with a light/dark regime of 13/11 at ~100uM light.  With the regime you are running the cultures may not be getting enough light.

Q:       Do you agitate the cultures at all? 

A:       We do not agitate cultures unless we reach volumes greater than 20L, and then they are agitated with air through an inline filter.