Thalassiosira pseudonana (Hustedt) Hasle et Heimdal
| Common Name | centric diatom | ||||
| Collection Site | 40.756°N -72.82°W Moriches Bay, Forge River, Long Island, New York USA | ||||
| Ocean | North Atlantic | ||||
| Sea | |||||
| Nearest Continent | North America | ||||
| Collected By | Guillard,R | ||||
| Collection Date | |||||
| Isolated By | Guillard,R | ||||
| Isolated Date | 09/08/1958 | ||||
| Identified By | |||||
| Deposited By | Guillard,R (WHOI) | ||||
| Deposit Date | 09/08/1958 | ||||
| Strain Synonyms | 3H, NEPCC58, CS173, CCAP 1085/12 | ||||
| Is The Strain Currently Axenic? | Yes | ||||
| When Was It Last Tested? | 01/05/2026 | ||||
| Other Information | estuarine:shallow embayments south shore Long Island.18%.,20C.; genome sequenced | ||||
| Authentic Type/Strain | No | ||||
| Morphological Data | |||||
| Attributes | Algae, Marine, Robust, High Lipid, Temperate, Aquaculture | ||||
| Additional Resources | Genbank Genome Transcriptome AlgaeBase | ||||
| Genome Sequence Link | Yes | ||||
| Medium Used for Maintenance | L1 |
| Other Reported Growth Media | ASM4, ASP6, ASP8a, f/2, f/2 agar, L1 agar, WC |
| Maintenance Temperature (°C) | 14 °C |
| Known Temperature Range (°C) | 4 - 25 °C |
| Cell Length (Min) | 4 |
| Cell Length (Max) | 6 |
| Cell Width (Min) | 4 |
| Cell Width (Max) | 5 |
CCMP1335 was cryopreserved on Aug 22 2000 using 12% DMSO as a cryoprotectant.
The time required to regrow this culture, prior shipping, is approximately 25 days. If interested, please contact the CCMP for the cryopreservation methods (freezing and/or thawing protocols).
Note that aquaculture strains are always maintained as actively growing cultures, even if also cryogenically stored. Therefore, aquaculture strain starter cultures (2x15ml) and only starter cultures can be shipped within 24h of ordering (see aquaculture express ordering on the CCMP home page).
Thalassiosira pseudonana genomic DNA containing 18S rRNA gene, ITS1, 5.8S rRNA gene, ITS2 and 28S rRNA gene, strain CCMP 1335
https://www.ncbi.nlm.nih.gov/nuccore/HF565133.1
Genome
https://www.ncbi.nlm.nih.gov/genome/54
Thalassiosira pseudonana strain CCMP 1335 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence
https://www.ncbi.nlm.nih.gov/nuccore/EF208793.1
A role for manganese in superoxide dismutases and growth of iron deficient diatoms
https://aslopubs.onlinelibrary.wiley.com/doi/abs/10.4319/lo.2004.49.5.1774
Extracellular production of superoxide by marine diatoms: contrasting effects on iron redox chemistry and bioavailability
https://aslopubs.onlinelibrary.wiley.com/doi/epdf/10.4319/lo.2005.50.4.1172
Assay optimization and regulation of urease activity in two marine diatoms
https://onlinelibrary.wiley.com/doi/abs/10.1046/j.1529-8817.2000.99037.x
New daughter set created every four weeks
Daughter set……….…25umol quanta m-2 s-1
Mother set………….….13umol quanta m-2 s-1
Grandmother set….….7umol quanta m-2 s-1
Relevant Publications:
Analysis of Autophagy Genes in Microalgae: Chlorella as a Potential Model to Study Mechanism of Autophagy (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3407069/)
High variability in cellular stoichiometry of carbon, nitrogen, and phosphorus within classes of marine eukaryotic phytoplankton under sufficient nutrient concentrations (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5880891/)
Trait-dependent variability of the response of marine phytoplankton to oil and dispersant exposure (https://www.sciencedirect.com/science/article/pii/S0025326X20300242)
The metabolite dimethylsulfoxonium propionate extends the marine organosulfur cycle (https://www.nature.com/articles/s41586-018-0675-0)
The model marine diatom Thalassiosira pseudonana likely descended from a freshwater ancestor in the genus Cyclotella (https://bmcecolevol.biomedcentral.com/articles/10.1186/1471-2148-11-125)
Linear discriminant analysis of single-cell fluorescence excitation spectra of five phytoplankton species (https://journals.sagepub.com/doi/10.1366/11-06294)
Silicon coordinates DNA replication with transcription of the replisome factors in diatom algae (https://link.springer.com/article/10.1007/s11105-018-1074-2)
A re-investigation of Sarcinochrysis marina (Sarcinochrysidales, Pelagophyceae) from its type locality and the descriptions of Arachnochrysis, Pelagospilus, Sargassococcus and Sungminbooa genera nov. (https://pubmed.ncbi.nlm.nih.gov/29427838/)
Effects of chrysolaminarin synthase knockdown in the diatom Thalassiosira pseudonana: Implications of reduced carbohydrate storage relative to green algae (https://www.sciencedirect.com/science/article/pii/S2211926416303964)
The influence of marine microbial activities on aerosol production: A laboratory mesocosm study (https://agupubs.onlinelibrary.wiley.com/doi/full/10.1002/2015JD023469)
Potential of lipid metabolism in marine diatoms for biofuel production (https://pubmed.ncbi.nlm.nih.gov/25763104/)
Preferential utilization of inorganic polyphosphate over other bioavailable phosphorus sources by the model diatoms Thalassiosira spp. (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6849833/)
Nutrient temperature and light stress alter phosphorus and carbon forms in culture-grown algae (https://www.sciencedirect.com/science/article/pii/S0304420310000253)
Experimental evolution of phytoplankton fatty acid thermal reaction norms (https://onlinelibrary.wiley.com/doi/full/10.1111/eva.12798)
The transcriptome and proteome of the diatom Thalassiosira pseudonana reveal a diverse phosphorus stress response (https://pubmed.ncbi.nlm.nih.gov/22479440/)
Dimethylsulfoxide reduction activity is linked to nutrient stress in Thalassiosira pseudonana NCMA 1335 (https://www.int-res.com/abstracts/meps/v507/p31-38/)
Methanesulfonate supports growth as the sole sulfur source for the marine diatom Thalassiosira pseudonana NCMA 1335 (https://www.researchgate.net/publication/311689843_Methanesulfonate_supports_growth_as_the_sole_sulfur_source_for_the_marine_diatom_Thalassiosira_pseudonana_NCMA_1335)
Thalassiosira spp. community composition shifts in response to chemical and physical forcing in the northeast Pacific Ocean (https://pubmed.ncbi.nlm.nih.gov/24065961/)
Competitive dynamics in two species of marine phytoplankton under non-equilibrium conditions (https://www.researchgate.net/publication/270039354_Competitive_dynamics_in_two_species_of_marine_phytoplankton_under_non-equilibrium_conditions)
Application of PTR-MS to an incubation experiment of the marine diatom Thalassiosira pseudonana (https://www.researchgate.net/publication/275822572_Application_of_PTR-MS_to_an_incubation_experiment_of_the_marine_diatom_Thalassiosira_pseudonana)
The response of exposure to increased CO2 and decreased pH Thalassiosira pseudonana to long-term (https://pubmed.ncbi.nlm.nih.gov/22053201/)
Glycosylceramides from marine green microalga Tetraselmis sp. (https://pubmed.ncbi.nlm.nih.gov/23089133/)
Gene biomarkers in diatom Thalassiosira pseudonana exposed to polycyclic aromatic hydrocarbons from contaminated marine surface sediments (https://pubmed.ncbi.nlm.nih.gov/21087797/)
The response of diatom central carbon metabolism to nitrogen starvation is different from that of green algae and higher plants (https://pubmed.ncbi.nlm.nih.gov/22065419/)
Influence of light on particulate organic matter utilization by attached and free-living marine bacteria (https://www.frontiersin.org/articles/10.3389/fmicb.2019.01204/full)
Use of agent-based modeling to explore the mechanisms of intracellular phosphorus heterogeneity in cultured phytoplankton (https://pubmed.ncbi.nlm.nih.gov/23666327/)
Identifying reference genes with stable expression from high throughput sequence data (https://pubmed.ncbi.nlm.nih.gov/23162540/)
A genome-scale metabolic model of Thalassiosira pseudonana CCMP 1335 for a systems-level understanding of its metabolism and biotechnological potential (https://pubmed.ncbi.nlm.nih.gov/32932853/)
A stress-induced protein associated with the girdle band region of the diatom Thalassiosira pseudonana (Bacillariophyta) (https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1529-8817.2005.00076.x)
Diatom plastids depend on nucleotide import from the cytosol (https://www.pnas.org/doi/full/10.1073/pnas.0808862106)
Localization and role of manganese superoxide dismutase in a marine diatom (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1676035/)
Synergistic effects of light, temperature, and nitrogen source on transcription of genes for carbon and nitrogen metabolism in the centric diatom Thalassiosira pseudonana (Bacillariophyceae) (https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1529-8817.2005.00139.x)
Marine phytoplankton temperature versus growth responses from polar to tropical waters – outcome of a scientific community-wide study (https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0063091)
Physiological response of 10 phytoplankton species exposed to macondo oil and the dispersant, Corexit (https://pubmed.ncbi.nlm.nih.gov/29464721/)
Dissolved organic phosphorus utilization by phytoplankton reveals preferential degradation of polyphosphates over phosphomonoesters (https://www.frontiersin.org/articles/10.3389/fmars.2018.00380/full)
Allelopathic inhibition of competing phytoplankton by North American strains of the toxic dinoflagellate, Alexandrium fundyense: Evidence from field experiments, laboratory experiments, and bloom events (https://www.sciencedirect.com/science/article/abs/pii/S1568988311000904)
Functional group-specific traits drive phytoplankton dynamics in the oligotrophic ocean (https://www.pnas.org/doi/10.1073/pnas.1518165112)
Sinking towards destiny: High throughput measurement of phytoplankton sinking rates through time-resolved fluorescence plate spectroscopy (https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0185166)
Enhanced formation of transparent exopolymer particles (TEP) under turbulence during phytoplankton growth (https://academic.oup.com/plankt/article/41/3/349/5506785)
Photosystem II protein clearance and FtsH function in the diatom Thalassiosira pseudonana (https://pubmed.ncbi.nlm.nih.gov/23504483/)
Phytoplankton-derived zwitterionic gonyol and dimethylsulfonioacetate interfere with microbial dimethylsulfoniopropionate sulfur cycling (https://pubmed.ncbi.nlm.nih.gov/32113191/)
Single-cell and bulk fluorescence excitation signatures of seven phytoplankton species during nitrogen depletion and resupply (https://journals.sagepub.com/doi/full/10.1177/0003702818812090)
Efficiency of the CO2-concentrating mechanism of diatoms (https://pubmed.ncbi.nlm.nih.gov/21321195/)
Best practices in the flow cytometry of microalgae (https://onlinelibrary.wiley.com/doi/full/10.1002/cyto.a.24328)
Effect of dead phytoplankton cells on the apparent efficiency of photosystem II (https://www.int-res.com/abstracts/meps/v382/p35-40/)
Remineralization of bioavailable iron by a heterotrophic dinoflagellate (https://www.researchgate.net/publication/230561452_Remineralization_of_bioavailable_iron_by_a_heterotrophic_dinoflagellate)
Ammonium uptake and growth limitation in marine phytoplankton (https://aslopubs.onlinelibrary.wiley.com/doi/abs/10.4319/lo.2007.52.6.2496)
A trace metal clean reagent to remove surface-bound iron from marine phytoplankton (https://www.sciencedirect.com/science/article/pii/S0304420303000549)
Influence of low light and a light:dark cycle on NO3- uptake, intracellular NO3- and nitrogen isotope fractionation by marine phytoplankton (https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1529-8817.2004.03171.x)
Chitin in diatoms and its association with the cell wall (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2708456/)
The effect of light stress on the release of volatile iodocarbons by three species of marine macroalgae (https://aslopubs.onlinelibrary.wiley.com/doi/10.4319/lo.2006.51.6.2849)
Nitric oxide as a signaling factor to upregulate the death-specific protein in a marine diatom, Skeletonema costatum, during blockage of electron flow in photosynthesis (https://pubmed.ncbi.nlm.nih.gov/18776028/)
Nickel limitation and zinc toxicity in a urea-grown diatom (https://aslopubs.onlinelibrary.wiley.com/doi/abs/10.4319/lo.2008.53.6.2462)
Extracellular production of superoxide by marine diatoms: contrasting effects on iron redox chemistry and bioavailability (https://aslopubs.onlinelibrary.wiley.com/doi/abs/10.4319/lo.2005.50.4.1172)
Assay optimization and regulation of urease activity in two marine diatoms (https://pubmed.ncbi.nlm.nih.gov/29544013/)
A role for manganese in superoxide dismutases and growth of iron deficient diatoms (https://aslopubs.onlinelibrary.wiley.com/doi/abs/10.4319/lo.2004.49.5.1774)
Copper requirements for iron acquisition and growth of coastal ad oceanic diatoms (https://aslopubs.onlinelibrary.wiley.com/doi/abs/10.4319/lo.2005.50.4.1149)
Lipid and fatty acid composition of diatoms revisited: rapid wound-activated change of food quality parameters influences herbivorous copepod reproductive success (https://pubmed.ncbi.nlm.nih.gov/17541989/)
The diatom EST database (https://academic.oup.com/nar/article/33/suppl_1/D344/2505429?login=false)
Interspecific differences in the bioconcentration of selenite by phytoplankton and their ecological implications (https://www.int-res.com/abstracts/meps/v213/p1-12/)
Nutritional properties of microalgae for mariculture (https://www.sciencedirect.com/science/article/abs/pii/S0044848696015013?via%3Dihub)
The effect of nutrient availability and temperature on chain length of the diatom, Skeletonema costatum (https://academic.oup.com/plankt/article/28/9/831/1589539)