Thalassiosira pseudonana (Hustedt) Hasle et Heimdal
|Common Name||centric diatom|
|Collection Site||40.756°N -72.82°W |
Moriches Bay, Forge River, Long Island, New York USA
|Nearest Continent||North America|
|Deposited By||Guillard,R (WHOI)|
|Strain Synonyms||3H, NEPCC58, CS173, CCAP 1085/12|
|Is The Strain Currently Axenic?||Yes|
|When Was It Last Tested?||09/07/2021|
|Other Information||estuarine:shallow embayments south shore Long Island.18%.,20C.; genome sequenced|
|Attributes||Algae, Marine, Robust, High Lipid, Temperate, Aquaculture|
|Medium Used for Maintenance||L1|
|Other Reported Growth Media||ASM4, ASP6, ASP8a, f/2, f/2 agar, L1 agar, WC|
|Maintenance Temperature (°C)||14 °C|
|Known Temperature Range (°C)||4 - 25 °C|
|Cell Length (Min)||4|
|Cell Length (Max)||6|
|Cell Width (Min)||4|
|Cell Width (Max)||5|
The time required to regrow this culture, prior shipping, is approximately 25 days. If interested, please contact the CCMP for the cryopreservation methods (freezing and/or thawing protocols).
Note that aquaculture strains are always maintained as actively growing cultures, even if also cryogenically stored. Therefore, aquaculture strains (see aquaculture express ordering on the CCMP home page) can be shipped immediately upon request.
Q: I would like to obtain a Thlassiosira pseudonana strain I can culture at 15°C. Do you have recommendation for a strain that is available?
A: I would recommend CCMP1335. It is axenic. It grows well. It is well documented (the genome has been sequenced)
Q: I have received CCMP1335 and have generated tansformants by using it. I am interested in the method of cryopreservation for the generated transformants.
A: Here is what we have in the database for cryopreservation of CCMP1335. This flask was started on 7Aug00 at 14degrees in f/2 medium. Before dispensing into cryovials, the overlying media was pipetted off to concentrate the cells.The cells were exposed to 12% of a 30% primary DMSO cryoprotectant for approx 24 minutes before the fullsw program was started. The probe was in the same final volume as the cells (1ml) and consisted of 5% DMSO diluted in f/2-si. Three test vials were also frozen. The three test vials were thawed on 22Aug00 at 36 degrees into 25ml tubes of room temperature f/2 medium. They were grown at 14 degrees in high light. By 29Aug they were just starting to grow, and by 6Sep all thaws were growing. By 13Sep, all three were almost shippable at approx half the density of the control. By 20 Aug, all three thaws were great. . The bact test showed the thaws to be axenic.
Q: I’d just like to know if they will survive if they are put them in a lab fridge an for how long they can be left unattended for in that fridge?
A: CCMP1335 has a known temp range of 4C to 25C for us. If the lab fridge has the correct lighting, soft white fluorescent bulbs at ~80uE, and a 13/11 light dark cycle the culture may be ok for a couple weeks.