Thalassiosira pseudonana  (Hustedt) Hasle et Heimdal

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$18.00 to $875.00
SKU: CCMP1335
Class: Mediophyceae

Grown to Order

Common Namecentric diatom
Collection Site40.756°N -72.82°W
Moriches Bay, Forge River, Long Island, New York USA
OceanNorth Atlantic
Sea
Nearest ContinentNorth America
Collected ByGuillard,R
Collection Date
Isolated ByGuillard,R
Isolated Date09/08/1958
Identified By
Deposited ByGuillard,R (WHOI)
Deposit Date09/08/1958
Strain Synonyms3H, NEPCC58, CS173, CCAP 1085/12
Is The Strain Currently Axenic?Yes
When Was It Last Tested?09/07/2021
Other Informationestuarine:shallow embayments south shore Long Island.18%.,20C.; genome sequenced
Authentic Type/StrainNo
Morphological Data
AttributesAlgae, Marine, Robust, High Lipid, Temperate, Aquaculture
Additional ResourcesGenbank
Algaebase
Medium Used for MaintenanceL1
Other Reported Growth MediaASM4, ASP6, ASP8a, f/2, f/2 agar, L1 agar, WC
Maintenance Temperature (°C)14 °C
Known Temperature Range (°C)4 - 25 °C
Cell Length (Min)4
Cell Length (Max)6
Cell Width (Min)4
Cell Width (Max)5
CCMP1335 was cryopreserved on Aug 22 2000 using 12% DMSO as a cryoprotectant.

The time required to regrow this culture, prior shipping, is approximately 25 days. If interested, please contact the CCMP for the cryopreservation methods (freezing and/or thawing protocols).

Note that aquaculture strains are always maintained as actively growing cultures, even if also cryogenically stored. Therefore, aquaculture strains (see aquaculture express ordering on the CCMP home page) can be shipped immediately upon request.

Q:       I would like to obtain a Thlassiosira pseudonana strain I can culture at 15°C. Do you have recommendation for a strain that is available?

A:       I would recommend CCMP1335It is axenic.  It grows well.  It is well documented (the genome has been sequenced)

Q:       I have received CCMP1335 and have generated tansformants by using it. I am interested in the method of cryopreservation for the generated transformants. 

A:       Here is what we have in the database for cryopreservation of CCMP1335.  This flask was started on 7Aug00 at 14degrees in f/2 medium. Before dispensing into cryovials, the overlying media was pipetted off to concentrate the cells.The cells were exposed to 12% of a 30% primary DMSO cryoprotectant for approx 24 minutes before the fullsw program was started. The probe was in the same final volume as the cells (1ml) and consisted of 5% DMSO diluted in f/2-si.  Three test vials were also frozen.  The three test vials were thawed on 22Aug00 at 36 degrees into 25ml tubes of  room temperature f/2  medium.  They were grown at 14 degrees in high light. By 29Aug they were just starting to grow, and by 6Sep all thaws were  growing. By 13Sep, all three were almost shippable at approx half the density of the control. By 20 Aug, all three thaws were great. . The bact test showed the thaws to be axenic.

Q:       I’d just like to know if they will survive if they are put them in a lab fridge an for how long they can be left unattended for in that fridge?

A:       CCMP1335 has a known temp range of 4C to 25C for us.  If the lab fridge has the correct lighting, soft white fluorescent bulbs at ~80uE, and a 13/11 light dark cycle the culture may be ok for a couple weeks.

 

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