Thalassiosira weissflogii

New daughter set created every four weeks

Daughter set……….…25umol quanta m-2 s-1

Mother set………….….13umol quanta m-2 s-1

Grandmother set….….7umol quanta m-2 s-1

Q+A:

Q: We are going to buy 1 liter from the strain: CCMP1051 Thalassiosira weissflogii (Grun.) Fryxell et Hasle. What are the conditions to maintain axenic the strain and what nutrients and concentration?

A: CCMP 1051 is kept axenic by sterile technique under a Laminar Flow hood. Click herefor the L1 media recipe.

Q: If I put 2 vials in the freezer, would that keep for 2 months without cell quality interruptions?

A: Without following a cryopreservation protocol that includes a -80C freezer, liquid nitrogen vapor and a cryoprotective agent I wouldn't recommend freezing cultures. Ice formation disrupts lipid bilayers and will destroy the viability of the culture.

Q: We were wondering if you may have nutritional profile?

A: I am afraid that is not information that we have.

Documentation:

Cultivation of the Marine Pelagic Tunicate Dolioletta gegenbauri (Uljanin 1884) for Experimental Studies (https://pubmed.ncbi.nlm.nih.gov/31449255/)

Physiological adjustments and transcriptome reprogramming are involved in the acclimation to salinity gradients in diatoms (https://pubmed.ncbi.nlm.nih.gov/27236063/)

Nutrient temperature and light stress alter phosphorus and carbon forms in culture-grown algae (https://www.sciencedirect.com/science/article/pii/S0304420310000253)

Estimation of chromophoric dissolved organic matter (CDOM) and photosynthetic activity of estuarine phytoplankton using a multiple-fixed- wavelength spectral fluorometer (https://www.sciencedirect.com/science/article/pii/S0043135412009104)

Marine sources of ice nucleating particles: results from phytoplankton cultures and samples collected at sea (https://ui.adsabs.harvard.edu/abs/2016AGUFM.A51F0126W/abstract)

New tools for labeling silica in living diatoms (https://pubmed.ncbi.nlm.nih.gov/18069957/)