Mediolabrus comicus (H. Takano) Yang Li
| Common Name | |||||
| Taxonomy by Gene Sequence | No | ||||
| Collection Site | 30.397173°N -86.515876°W Choctawhatchee Bay, Destin, FL, USA. Plankton tow from Destin Marina. | ||||
| Ocean | |||||
| Sea | |||||
| Nearest Continent | |||||
| Collected By | Andrew Alverson | ||||
| Collection Date | |||||
| Isolated By | Andrew Alverson | ||||
| Isolated Date | 07/25/2014 | ||||
| Identified By | |||||
| Deposited By | Elizabeth Ruck | ||||
| Deposit Date | 10/11/2022 | ||||
| Strain Synonyms | AJA131-32 | ||||
| Is The Strain Currently Axenic? | No | ||||
| When Was It Last Tested? | |||||
| Other Information | |||||
| Authentic Type/Strain | No | ||||
| Morphological Data | |||||
| Attributes | Algae, Marine, Warm Water | ||||
| Additional Resources | Genbank Genome Transcriptome AlgaeBase | ||||
| Genome Sequence Link | No | ||||
| Medium Used for Maintenance | L1 |
| Other Reported Growth Media | |
| Maintenance Temperature (°C) | 20 °C |
| Known Temperature Range (°C) | 20 - 21 °C |
| Cell Length (Min) | |
| Cell Length (Max) | |
| Cell Width (Min) | |
| Cell Width (Max) |
This lot is a result of a subculture made 5Jan2024 in L1+ medium at 20º C. After removing about 10 mL of overlying medium, the remaining culture was mixed, and 9mL of that was mixed with 6mL of 30% CPA They were exposed to cryoprotectant for 20 minutes before the FSW program was started. Three test vials were also frozen.
The Fullsw Program is as follows:
Wait @ 4.0°C until space.
Rate@ 1.0°C/minute until sample =-9.0°C.
Rate@ 25.0°C/minute until chamber =-45.0°C.
Rate@ 15.0°C/minute until chamber =-25.0°C.
Rate@ 1.0°C/minute until chamber =-45.0°C.
Rate@ 10.0°C/minute until chamber =-90.0°C.
END
Three test vials were thawed at 20º C until there was just a little ice left. They were then spun at 7000 rpm for approx. 10 seconds in an eppendorf centrifuge to pellet the cells. The supernantant was removed and the cells resuspended and pipetted into room temperature tubes of L1+. They were grown at 20º C in high light.The thaws were growing and shippable after 21 days.The bacto tests showed that the thaws were contaminated with bacteria as in the active collection. No fungal contamination was detected.
New daughter set created every two weeks
Daughter set……….…43umol quanta m-2 s-1
Mother set………….…16umol quanta m-2 s-1
Grandmother set….….13umol quanta m-2 s-1