Cyclotella choctawhatcheeana Prasad
| Common Name | |||||
| Taxonomy by Gene Sequence | No | ||||
| Collection Site | 43.479932°N -70.412892°W Saco River, Saco, ME, USA. Plankton tow from Marston's Marina. | ||||
| Ocean | |||||
| Sea | |||||
| Nearest Continent | |||||
| Collected By | Kala Downey | ||||
| Collection Date | |||||
| Isolated By | Kala Downey | ||||
| Isolated Date | 10/20/2017 | ||||
| Identified By | |||||
| Deposited By | Elizabeth Ruck | ||||
| Deposit Date | 10/11/2022 | ||||
| Strain Synonyms | AJA259-04 | ||||
| Is The Strain Currently Axenic? | No | ||||
| When Was It Last Tested? | |||||
| Other Information | |||||
| Authentic Type/Strain | No | ||||
| Morphological Data | |||||
| Attributes | Algae, Marine, Cold Water | ||||
| Additional Resources | Genbank Genome Transcriptome AlgaeBase | ||||
| Genome Sequence Link | No | ||||
| Medium Used for Maintenance | 35% |
| Other Reported Growth Media | |
| Maintenance Temperature (°C) | 14 °C |
| Known Temperature Range (°C) | 14 - 15 °C |
| Cell Length (Min) | |
| Cell Length (Max) | |
| Cell Width (Min) | |
| Cell Width (Max) |
This lot is a result of a subculture made in 35% medium at 14º C. Approx. 5mL of overlying medium was removed, the remaining culture mixed, and then 9mL of that was mixed with 6mL of 30% CPA. 1mL of that was dispensed into each cryovial. The vials were exposed to cryoprotectant for 27 minutes before the fullsw program was started. The probe was in a final volume of 1ml and consisted of 10% DMSO diluted in 35%. Three test vials were also frozen.
Three test vials were thawed at 20º C until there was just a little ice left. They were then spun at 7000 rpm for approx. 10 seconds in an eppendorf centrifuge to pellet the cells. The supernantant was removed and the cells resuspended and pipetted into room temperature tubes of 35%. They were grown at 14º C in high light. The thaws were growing and shippable after 28 days.The bacto tests showed that the thaws were contaminated with bacteria as in the active collection. No fungal contamination was detected.