Thalassiosira oceanica  (Hustedt) Hasle et Heimdal

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$18.00 to $875.00
SKU: CCMP998
Class: Mediophyceae

Grown to Order

Common Namecentric diatom
Collection Site39.0833°N -71.9333°W
continental slope
OceanNorth Atlantic
Sea
Nearest ContinentNorth America
Collected ByMurphy,L
Collection Date
Isolated ByMurphy,L
Isolated Date
Identified By
Deposited ByMurphy,L
Deposit Date01/04/1985
Strain SynonymsK58-101, 58-101
Is The Strain Currently Axenic?No
When Was It Last Tested?05/23/1995
Other Informationwarm core eddy
Authentic Type/StrainNo
Morphological Data
AttributesAlgae, Marine
Additional ResourcesGenbank
Algaebase
Medium Used for MaintenanceL1
Other Reported Growth Mediaf/2
Maintenance Temperature (°C)20 °C
Known Temperature Range (°C)18 - 26 °C
Cell Length (Min)8
Cell Length (Max)14
Cell Width (Min)6
Cell Width (Max)8
CCMP998 was cryopreserved on Mar 6 2019 using 12% DMSO as a cryoprotectant.

The time required to regrow this culture, prior shipping, is approximately 28 days. If interested, please contact the CCMP for the cryopreservation methods (freezing and/or thawing protocols).

Note that aquaculture strains are always maintained as actively growing cultures, even if also cryogenically stored. Therefore, aquaculture strains (see aquaculture express ordering on the CCMP home page) can be shipped immediately upon request.

Q:       You mentioned that these cultures were cryopreserved before we received them. On the Bigelow homepage, I found some information about the cryopreservation procedure, but not a detailed protocol. Is there such a protocol that you could share with us?  We would especially like to know how dense cultures should be for cryopreservation as well as which survival rates one can expect.

A:       We try to cryo strains when they reach mid log so every culture is different.  We run a control with every cryo event of three tubes.  These are pulled twenty-four hours after the event has been placed in vapor LN2.  Only if all three tubes establish viable biomass is the cryo run deemed successful.  Once the culture has reached mid log it is mixed with the cryoprotective agent(CPA), DMSO is this case.  The percentage and grow back time is listed for the strains you ordered on the webpage under the "Cryopreservation" tab.  A minimum of fifteen minutes in the CPA before we aliquot 1mL into cryo tubes.  These are then placed in a "Mr Frosty" and stored at -80 for at least three hours.  The cryo tubes are then placed in LN2  vapor for long term storage.

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