|Common Name||blue-green algae|
|Collection Site||43.8441°N -69.6413°W |
Bigelow Laboratory dock, West Boothbay Harbor, Maine USA
|Sea||Gulf of Maine|
|Nearest Continent||North America|
|Strain Synonyms||6B3, 6B3SYN|
|Is The Strain Currently Axenic?||No|
|When Was It Last Tested?||04/30/1999|
|Attributes||Algae, Marine, Temperate|
|Medium Used for Maintenance||L1 - Si|
|Other Reported Growth Media||f/2-Si, L1 - Si, SNTR agar|
|Maintenance Temperature (°C)||14 °C|
|Known Temperature Range (°C)||11 - 16 °C|
|Cell Length (Min)||2|
|Cell Length (Max)||2|
|Cell Width (Min)||1|
|Cell Width (Max)||1|
The time required to regrow this culture, prior shipping, is approximately 25 days. If interested, please contact the CCMP for the cryopreservation methods (freezing and/or thawing protocols).
Note that aquaculture strains are always maintained as actively growing cultures, even if also cryogenically stored. Therefore, aquaculture strains (see aquaculture express ordering on the CCMP home page) can be shipped immediately upon request.
Q: As part of an ongoing project, I want to use flow cytometry techniques to sort and analyze algal cells. I was wondering about your Synechococcus cultures because I am currently trying to make a mock community containing diatoms, dinoflagellates, coccolithophores, chlorophytes, and cyanobacteria for flow cytometry. Since our cells (mostly isolated from the Pacific) are stored in a 12C fridge, I was looking into your CCMP835 isolate that is stored in 14C. Its description states that it is cryopreserved, and I am wondering if it is still possible to incorporate this strain into our current mock community just for the sake of flow cytometry analysis? If it is convenient, I'd like to learn more about your Synechococcus strains and understand how to thaw and culture them. I would also be happy to answer any questions you have regarding my plan for the project. Any input and advice is greatly appreciated.
A: I have 1 culture that may be of interest to you. It fits within the 12C parameter and is not cryopreserved. I have enclosed and Excel with relevant information. CCMP 835 was collected off the dock at Bigelow in West Boothbay Harbor so I am going on the precedent that collection site is not as important as temperature range.