New daughter set created every three weeks
Daughter set……….…26umol quanta m-2 s-1
Mother set………….…20umol quanta m-2 s-1
Grandmother set….….10umol quanta m-2 s-1
Q: Is this culture maintained in ~32psu seawater, grows quickly and densely?
A: CCMP426 is maintained in ~32psu(L1-Si). It is reinoculated every 3 weeks and will get dense by 9 weeks. Once you have a healthy culture you may be able to increase the density before 9 weeks.
Q:Are you maintaining it at 20C? If so, do you suspect that it would grow faster at temps between 23 and 26 C?
A:We maintain at 20, yes. I have a max temp range of 26 in the records, should be ok.
Use of fluorescein diacetate (FDA) as a single-cell probe of metabolic activity in dinoflagellate cultures (https://www.tandfonline.com/doi/abs/10.1080/01965581.1988.10749548)