Karlodinium veneficum (D. Ballantine) J. Larsen
| Common Name | alveolates | ||||
| Taxonomy by Gene Sequence | No | ||||
| Collection Site | 25.6°N -81°W Florida Everglades, Florida USA (lat long approximate) | ||||
| Ocean | North Atlantic | ||||
| Sea | Caribbean Sea | ||||
| Nearest Continent | North America | ||||
| Collected By | Dodson,A | ||||
| Collection Date | |||||
| Isolated By | Dodson,A | ||||
| Isolated Date | 11/01/1965 | ||||
| Identified By | Place,A | ||||
| Deposited By | Thomas,W | ||||
| Deposit Date | 05/12/1982 | ||||
| Strain Synonyms | WTFLA | ||||
| Is The Strain Currently Axenic? | No | ||||
| When Was It Last Tested? | 12/20/2006 | ||||
| Other Information | |||||
| Authentic Type/Strain | No | ||||
| Morphological Data | |||||
| Attributes | Algae, Toxic, Marine, Warm Water | ||||
| Additional Resources | Genbank Genome Transcriptome AlgaeBase | ||||
| Genome Sequence Link | No | ||||
| Medium Used for Maintenance | L1-Si |
| Other Reported Growth Media | f/2-Si, K, L1 - Si, Prov |
| Maintenance Temperature (°C) | 20 °C |
| Known Temperature Range (°C) | 20 - 26 °C |
| Cell Length (Min) | 10 |
| Cell Length (Max) | 15 |
| Cell Width (Min) | 8 |
| Cell Width (Max) | 12 |
New daughter set created every three weeks
Daughter set……….…26umol quanta m-2 s-1
Mother set………….…20umol quanta m-2 s-1
Grandmother set….….10umol quanta m-2 s-1
Q+A:
Q: Is this culture maintained in ~32psu seawater, grows quickly and densely?
A: CCMP426 is maintained in ~32psu(L1-Si). It is reinoculated every 3 weeks and will get dense by 9 weeks. Once you have a healthy culture you may be able to increase the density before 9 weeks.
Q:Are you maintaining it at 20C? If so, do you suspect that it would grow faster at temps between 23 and 26 C?
A:We maintain at 20, yes. I have a max temp range of 26 in the records, should be ok.
Documentation:
Use of fluorescein diacetate (FDA) as a single-cell probe of metabolic activity in dinoflagellate cultures (https://www.tandfonline.com/doi/abs/10.1080/01965581.1988.10749548)