Thalassiosira allenii H.Takano
| Common Name | |||||
| Taxonomy by Gene Sequence | No | ||||
| Collection Site | 29.886182°N -81.285332°W Salt Run, St. Augustine, FL, USA. Plankton tow from pier near St. Augustine Lighthouse Park & St. Augustine Yacht Club | ||||
| Ocean | |||||
| Sea | |||||
| Nearest Continent | |||||
| Collected By | Elizabeth Ruck | ||||
| Collection Date | |||||
| Isolated By | Kala Downey | ||||
| Isolated Date | |||||
| Identified By | |||||
| Deposited By | Elizabeth Ruck | ||||
| Deposit Date | 10/11/2022 | ||||
| Strain Synonyms | AJA297-17 | ||||
| Is The Strain Currently Axenic? | No | ||||
| When Was It Last Tested? | |||||
| Other Information | |||||
| Authentic Type/Strain | No | ||||
| Morphological Data | |||||
| Attributes | Algae, Marine, Warm Water | ||||
| Additional Resources | Genbank Genome Transcriptome AlgaeBase | ||||
| Genome Sequence Link | No | ||||
| Medium Used for Maintenance | L1 |
| Other Reported Growth Media | |
| Maintenance Temperature (°C) | 20 °C |
| Known Temperature Range (°C) | 20 - 21 °C |
| Cell Length (Min) | |
| Cell Length (Max) | |
| Cell Width (Min) | |
| Cell Width (Max) |
This lot is a result of a subculture made in L1 medium at 20º C. Approx. 5mL of overlying medium was removed, the remaining culture mixed, and then 9mL of that was mixed with 6mL of 30% CPA. 1mL of that was dispensed into each cryovial. The vials were exposed to cryoprotectant for 21 minutes before the fullsw program was started. The probe was in a final volume of 1ml and consisted of 10% DMSO diluted in L1-Si. Three test vials were also frozen.
Three test vials were thawed at 20º C until there was just a little ice left. They were then spun at 5000 rpm for approx. 5 seconds in an eppendorf centrifuge to pellet the cells. The supernantant was removed and the cells resuspended and pipetted into room temperature tubes of L1. They were grown at 20º C in high light. The control was shippable after 15 days. The thaws were growing and shippable after 21 days.The bacto tests showed that the thaws were contaminated with bacteria as in the active collection. No fungal contamination was detected.
New daughter set created every two weeks
Daughter set……….…43umol quanta m-2 s-1
Mother set………….…16umol quanta m-2 s-1
Grandmother set….….13umol quanta m-2 s-1