Pelagomonas calceolata  Andersen et Saunders

This lot is a result of a subculture made 25Sept2024 in L1-Si medium at 20º C. Approx. 10mL of overlying medium was removed, the remaining culture mixed, then 12mL of that mixed culture was mixed with 3mL of 30% CPA. 1mL of that was dispensed into each cryovial. The vials were exposed to cryoprotectant for 20 minutes before the fullsw program was started. The probe was in a final volume of 1ml and consisted of 10% DMSO diluted in L1. Three test vials were also frozen.

Three test vials were thawed at 20º C until there was just a little ice left. They were then spun at 7000 rpm for approx. 10 seconds in an eppendorf centrifuge to pellet the cells. The supernantant was removed and the cells resuspended and pipetted into room temperature tubes of L1-Si. They were grown at 20º C in high light. The control was shippable after 20 days. The thaws were shippable after 30 days.The bacto tests showed that the thaws were contaminated with bacteria as in the active collection. No fungal contamination was detected.