Thalassiosira weissflogii (Grun.) Fryxell et Hasle
|Common Name||centric diatom|
|Collection Site||41.11°N -72.1°W |
Gardiners Island, Long Island, New York USA
|Sea||Long Island Sound|
|Nearest Continent||North America|
|Is The Strain Currently Axenic?||Yes|
|When Was It Last Tested?||06/15/2021|
|Morphological Data||single cells|
|Attributes||Algae, Marine, Robust, Temperate, Aquaculture|
|Medium Used for Maintenance||L1|
|Other Reported Growth Media||f/2|
|Maintenance Temperature (°C)||14 °C|
|Known Temperature Range (°C)||11 - 22 °C|
|Cell Length (Min)||12|
|Cell Length (Max)||22|
|Cell Width (Min)||10|
|Cell Width (Max)||12|
The time required to regrow this culture, prior shipping, is approximately 44 days. If interested, please contact the CCMP for the cryopreservation methods (freezing and/or thawing protocols).
Note that aquaculture strains are always maintained as actively growing cultures, even if also cryogenically stored. Therefore, aquaculture strains (see aquaculture express ordering on the CCMP home page) can be shipped immediately upon request.
Q: I was wondering whether you could share information about CCMP1336 their growth rates (in L1 medium). We are using it for a time-sensitive project and I would like to have a better understanding of which species had high growth rates.
A: CCMP333 is a fairly slow grower (3wks at 14C) so maybe 6-8 weeks to ship 1L. CCMP1336 and 1320 are aqua culture so could ship 1L in 2-3 weeks. The remainder are cryo preserved and will take 2-4 months to ship 1L.
Q: I have been maintaining CCMP1336 in small cultures in marine minimal media and L1 with a light/dark cycle of 8/16 with approx 30-50 umol m^-2 s^-1 of light and at ~18 °C. My cells seem to be losing their color and are mostly opaque now. When they arrived, they were mostly green. Do you have ideas how to get their pigment back.
A: We maintain cultures with a light/dark regime of 13/11 at ~100uM light. With the regime you are running the cultures may not be getting enough light.
Q: Do you agitate the cultures at all?
A: We do not agitate cultures unless we reach volumes greater than 20L, and then they are agitated with air through an inline filter.