|Common Name||blue-green algae|
|Collection Site||19.75°N -92.4167°W |
Gulf of Mexico Researcher 838
|Sea||Gulf of Mexico|
|Nearest Continent||North America|
|Deposited By||Brand,L and Waterbury,J|
|Strain Synonyms||838BG, WH8007|
|Is The Strain Currently Axenic?||No|
|When Was It Last Tested?||10/18/2006|
|Other Information||surface; green strain; maintained at CCMP in dim light|
|Attributes||Algae, Marine, Warm Water|
|Medium Used for Maintenance||L1 - Si|
|Other Reported Growth Media||ASM2, ASM3, ASM4, ASP8a, f/2 agar, f/2-Si, K, L1 - Si, SN, SNTR|
|Maintenance Temperature (°C)||24 °C|
|Known Temperature Range (°C)||22 - 26 °C|
|Cell Length (Min)||1|
|Cell Length (Max)||2|
|Cell Width (Min)||1|
|Cell Width (Max)||1|
The time required to regrow this culture, prior shipping, is approximately 0 days. If interested, please contact the CCMP for the cryopreservation methods (freezing and/or thawing protocols).
Note that aquaculture strains are always maintained as actively growing cultures, even if also cryogenically stored. Therefore, aquaculture strains (see aquaculture express ordering on the CCMP home page) can be shipped immediately upon request.
Q: Do you know if you have any Synechococcus that are green (lack phycoerythrin)?
A: CCMP836 and CCMP1333 are both green Synechococcus
Q: we need to grow up a couple of marine cyanos. Ideally multiple liters at 10 to the 6 per ml. The researchers have requested the following strains: Synechococcus sp. 836 Prochlorococcus marinus 2389 We can devote two incubators to the project, but any info on recommended light intensities for these strain will help with our considerations. Most importantly, given that we plan to meet your media and temperature recommendations, any potential growth rates would be very help
A: Both are grown on a 13:11 light cycle. CCMP836 is grown in dim light Approx 15 umol m2 S-1 CCMP 836 can tolerate a slightly higher light level when it is up to a higher volume(above 1L) this does tend to affect the pigments however. They end up looking washed out and pale. I do not have a growth curve on that one. CCMP2389 is grown in a higher light at 50-60 umol m2 S-1 . This organism has high nutrient needs It is grown in a modified pro99 with 2X N and P. It will crash quickly and spectacularly when it depletes its supply of nutrients. Below is a growth curve based on relative fluorescent units which you may find helpful. The starting volume was 30mls.