Proteomonas sulcata

New daughter set created every two weeks

Daughter set……….…33umol quanta m-2 s-1

Mother set………….….10umol quanta m-2 s-1

Grandmother set….….10umol quanta m-2 s-1

Q+A:

Q: I have a problem about culturing. Soon after arriving (July 29), the strains are diluted (2:1) and inoculated in Growth media for first transfer. Now they are incubated at preferable temperature for each strain. Also, I checked cultures with a microscope. I saw that in 704 some particles were moving but they were completely different (tiny, looks like black dots). I can’t judge whether this is good or not because this is my first time to culture them. Light-dark cycle is nearly 13:11 according to your protocol. Unfortunately, I could not prepare the incubator maintained at 2C. I think they are increased compared to the day of arriving. I would like ask you to help me about culturing these strains.

A: CCMP704 are non axenic . The black dots may be bacteria.

Documentation:

Nuclear and Nucleomorph SSU rDNA Phylogeny in the Cryptophyta and the evolution of Cryptophyte diversity (https://pubmed.ncbi.nlm.nih.gov/12107593/)

Phylogenetic relationships among Cryptophyta: Analysis of nuclear encoded SSU rRNA support the monophyly of extant plastic-containing lineages (https://pubmed.ncbi.nlm.nih.gov/23194638/)