Proteomonas sulcata Hill et Wetherbee
| Common Name | chromists | ||||
| Taxonomy by Gene Sequence | No | ||||
| Collection Site | collection site unknown | ||||
| Ocean | |||||
| Sea | |||||
| Nearest Continent | Unknown | ||||
| Collected By | |||||
| Collection Date | |||||
| Isolated By | Hill,DRA | ||||
| Isolated Date | |||||
| Identified By | Hill,DRA | ||||
| Deposited By | Hill,DRA | ||||
| Deposit Date | 12/01/1990 | ||||
| Strain Synonyms | Mel-008 | ||||
| Is The Strain Currently Axenic? | No | ||||
| When Was It Last Tested? | 09/29/2006 | ||||
| Other Information | |||||
| Authentic Type/Strain | No | ||||
| Morphological Data | |||||
| Attributes | Algae, Marine | ||||
| Additional Resources | Genbank Genome Transcriptome AlgaeBase | ||||
| Genome Sequence Link | No | ||||
| Medium Used for Maintenance | L1 - Si |
| Other Reported Growth Media | f/2-Si, L1 - Si |
| Maintenance Temperature (°C) | 20 °C |
| Known Temperature Range (°C) | 18 - 22 °C |
| Cell Length (Min) | 12 |
| Cell Length (Max) | 14 |
| Cell Width (Min) | 5 |
| Cell Width (Max) | 7 |
New daughter set created every two weeks at 20uL into 18mL
Daughter set……….…33umol quanta m-2 s-1
Mother set………….….10umol quanta m-2 s-1
Grandmother set….….10umol quanta m-2 s-1
Q+A:
Q: I have a problem about culturing. Soon after arriving (July 29), the strains are diluted (2:1) and inoculated in Growth media for first transfer. Now they are incubated at preferable temperature for each strain. Also, I checked cultures with a microscope. I saw that in 704 some particles were moving but they were completely different (tiny, looks like black dots). I can’t judge whether this is good or not because this is my first time to culture them. Light-dark cycle is nearly 13:11 according to your protocol. Unfortunately, I could not prepare the incubator maintained at 2C. I think they are increased compared to the day of arriving. I would like ask you to help me about culturing these strains.
A: CCMP704 are non axenic . The black dots may be bacteria.
Documentation:
Nuclear and Nucleomorph SSU rDNA Phylogeny in the Cryptophyta and the evolution of Cryptophyte diversity (https://pubmed.ncbi.nlm.nih.gov/12107593/)
Phylogenetic relationships among Cryptophyta: Analysis of nuclear encoded SSU rRNA support the monophyly of extant plastic-containing lineages (https://pubmed.ncbi.nlm.nih.gov/23194638/)