Proteomonas sulcata

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Q:       I have a problem about culturing.  Soon after arriving (July 29), the strains are diluted (2:1) and inoculated in Growth media for first transfer.  Now they are incubated at preferable temperature for each strain.  Also, I checked cultures with a microscope.  I saw that in 704 some particles were moving but they were completely different (tiny, looks like black dots).  I can’t judge whether this is good or not because this is my first time to culture them.  Light-dark cycle is nearly 13:11 according to your protocol.  Unfortunately, I could not prepare the incubator maintained at 2C.  I think they are increased compared to the day of arriving.  I would like ask you to help me about culturing these strains.

A:       CCMP704 are non axenic .  The black dots may be bacteria. 

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