Tisochrysis lutea Bendif et. Probert
| Common Name | golden algae | ||||
| Taxonomy by Gene Sequence | No | ||||
| Collection Site | 22°N -72°W Little Water Cay, Turks and Caicos Islands, British West Indies | ||||
| Ocean | North Atlantic | ||||
| Sea | Caribbean Sea | ||||
| Nearest Continent | North America | ||||
| Collected By | Glazer,R | ||||
| Collection Date | |||||
| Isolated By | Glazer | ||||
| Isolated Date | 08/03/1985 | ||||
| Identified By | |||||
| Deposited By | Davis,M | ||||
| Deposit Date | 11/19/1984 | ||||
| Strain Synonyms | CISO | ||||
| Is The Strain Currently Axenic? | Yes | ||||
| When Was It Last Tested? | 03/04/2026 | ||||
| Other Information | 3 kt current in channel, 28C, 36ppt,leeward thru Providenciales | ||||
| Authentic Type/Strain | No | ||||
| Morphological Data | |||||
| Attributes | Algae, Marine, Robust, High Lipid, Warm Water, Aquaculture | ||||
| Additional Resources | Genbank Genome Transcriptome AlgaeBase | ||||
| Genome Sequence Link | No | ||||
| Medium Used for Maintenance | L1 - Si |
| Other Reported Growth Media | f/2-Si, K, |
| Maintenance Temperature (°C) | 24 °C |
| Known Temperature Range (°C) | 17 - 30 °C |
| Cell Length (Min) | 4 |
| Cell Length (Max) | 8 |
| Cell Width (Min) | 4 |
| Cell Width (Max) | 6 |
CCMP463 was cryopreserved on Dec 2 1999 using 10% DMSO as a cryoprotectant.
The time required to regrow this culture, prior shipping, is approximately 21 days. If interested, please contact the CCMP for the cryopreservation methods (freezing and/or thawing protocols).
Note that aquaculture strains are always maintained as actively growing cultures, even if also cryogenically stored. Therefore, aquaculture strain starter cultures (2x15ml) and only starter cultures can be shipped within 24h of ordering (see aquaculture express ordering on the CCMP home page).
New daughter set created every three weeks at 10uL into 18mL
Daughter set……….…39umol quanta m-2 s-1
Mother set………….….8umol quanta m-2 s-1
Grandmother set….….16umol quanta m-2 s-1
Q+A:
Q: We ordered algae CCMP463 from your laboratories in 2014. The culture was growing well until recently. Sometimes the algae look one day fine, but they aggregate and settle down overnight. We grow our algae in artificial seawater with F1/2 medium under 14/10 day night conditions. We do airbubble in the culturing flasks (we use 1L or 2L bottles). Do you have any tip what can prevent aggregation of the algae?
A: I am afraid that we grow all our cultures in filtered natural seawater with a 13/11 light/dark cycle without aeration.
Documentation:
On the description of Tisochrysis lutea gen. nov. sp. nov. and Isochrysis nuda sp. nov. in the Isochrysidales, and the transfer of Dicrateria to the Prymnesiales (Haptophyta). (https://link.springer.com/article/10.1007/s10811-013-0037-0)
Ingestion size of food microalgae of the Pacific Oyster Crassostrea gigas larvae (http://malacol.or.kr/bbs/board.php?bo_table=ju_list&wr_id=431&page=30)
Digestion indices of 12 species of microalgae by the oyster Crassostrea gigas larval development stages (http://www.malacol.or.kr/bbs/board.php?bo_table=ju_list&wr_id=438&page=27)