Rhodomonas salina (Wislouch) Hill et Wetherbee
| Common Name | chromists | ||||
| Taxonomy by Gene Sequence | No | ||||
| Collection Site | -38.293°N 144.6°W Point Lonsdale, Victoria, Australia | ||||
| Ocean | Indian | ||||
| Sea | Bass Strait | ||||
| Nearest Continent | Australia | ||||
| Collected By | Hill,DRA | ||||
| Collection Date | 02/13/1981 | ||||
| Isolated By | Hill,DRA | ||||
| Isolated Date | |||||
| Identified By | Hill,DRA | ||||
| Deposited By | Hill,DRA | ||||
| Deposit Date | 12/01/1990 | ||||
| Strain Synonyms | Mel-031,JS876 | ||||
| Is The Strain Currently Axenic? | No | ||||
| When Was It Last Tested? | 09/29/2006 | ||||
| Other Information | Marine | ||||
| Authentic Type/Strain | No | ||||
| Morphological Data | |||||
| Attributes | Algae, Marine | ||||
| Additional Resources | Genbank Genome Transcriptome AlgaeBase | ||||
| Genome Sequence Link | No | ||||
| Medium Used for Maintenance | L1 - Si |
| Other Reported Growth Media | f/2-Si, L1 - Si |
| Maintenance Temperature (°C) | 20 °C |
| Known Temperature Range (°C) | 18 - 22 °C |
| Cell Length (Min) | 9 |
| Cell Length (Max) | 16 |
| Cell Width (Min) | 6 |
| Cell Width (Max) | 8 |
This culture was started 21May07 in a tube of L1+/50uM NH4Cl/0.075%agar medium at 20 degrees C.The culture was mixed and dispensed into cryovials. They were exposed to cryoprotectant for 25 minutes before the fullsw program was started. The probe was in a final volume of 1ml and consisted of 10% DMSO diluted in L1. Three test vials were also frozen.
Three test vials were thawed on 24May07 at 20 degrees just until the ice started to melt and the entire contents of the cryovials could be pipetted out and subcultured into a tube of room temperature L1 medium after a ~1 second low speed spin in an Eppendorf centrifuge to get all the liquid out of the cap .They were grown in box1 at 20 degrees C in high light. The control was almost shippable by 29May (was shippable in 10 days). One thaw was growing by 19Jun and two were shippable by 27Jun. The bacto tests showed that the thaws were contaminated with bacteria as in the active collection. No fungal contaminantion was detected.
Note that aquaculture strains are always maintained as actively growing cultures, even if also cryogenically stored. Therefore, aquaculture strains (see aquaculture express ordering on the CCMP home page) can be shipped immediately upon request.