Geminigera cryophila (D. L. Taylor & C.C. Lee) D.R.A.Hill
| Common Name | chromists | ||||
| Taxonomy by Gene Sequence | No | ||||
| Collection Site | -77.8333°N 163°W McMurdo Sound, Antarctica (lat long approximate) | ||||
| Ocean | Southern | ||||
| Sea | Ross Sea | ||||
| Nearest Continent | Antarctica | ||||
| Collected By | Stoecker, DK and Gustafson, DE | ||||
| Collection Date | |||||
| Isolated By | Stoecker,D and Johnson,M | ||||
| Isolated Date | |||||
| Identified By | Johnson,M | ||||
| Deposited By | Stoecker,DK and Johnson,MD | ||||
| Deposit Date | 07/06/2005 | ||||
| Strain Synonyms | |||||
| Is The Strain Currently Axenic? | No | ||||
| When Was It Last Tested? | 03/25/2014 | ||||
| Other Information | collected from a mixture of sea-ice and underlying water | ||||
| Authentic Type/Strain | No | ||||
| Morphological Data | |||||
| Attributes | Algae, Marine, Cold Water | ||||
| Additional Resources | Genbank Genome Transcriptome AlgaeBase | ||||
| Genome Sequence Link | No | ||||
| Medium Used for Maintenance | L1 - Si |
| Other Reported Growth Media | f/2-Si, L1 - Si |
| Maintenance Temperature (°C) | 2 °C |
| Known Temperature Range (°C) | 2 - 6 °C |
| Cell Length (Min) | 17 |
| Cell Length (Max) | 20 |
| Cell Width (Min) | 9 |
| Cell Width (Max) | 12 |
New daughter set created every five weeks
Daughter set……….…7umol quanta m-2 s-1
Mother set………….….10umol quanta m-2 s-1
Grandmother set….….7umol quanta m-2 s-1
Q+A:
Q: I have a problem about culturing. The strains are diluted (2:1) and inoculated in Growth media for first transfer. Now they are incubated at preferable temperature for each strain. Also, I checked cultures with a microscope. I saw that in cases of CCMP2564 some particles were moving but they were completely different (tiny, looks like black dots). I can’t judge whether this is good or not because this is my first time to culture them. Light-dark cycle is nearly 13:11 according to your protocol. Unfortunately, I could not prepare the incubator maintained at 2C. I think they are increased compared to the day of arriving. I would like ask you to help me about culturing these strains.
A: CCMP2564 are non axenic. The black dots may be bacteria.
Documentation:
Light capture and pigment diversity in marine and freshwater cryptophytes (https://onlinelibrary.wiley.com/doi/abs/10.1111/jpy.12816)