New daughter set created every eight weeks
Daughter set……….…5umol quanta m-2 s-1
Mother set………….….13umol quanta m-2 s-1
Grandmother set….….11umol quanta m-2 s-1
Q: Upon arrival, cultures were transferred into fresh media and after an initial pronounced decrease in cell concentration most of the strains seem now to be growing again. However, I would like to let you know that CCMP1330 does not seem to be doing very well at this moment. Upon arrival two cultures were made in a final volume of 30ml using a 5:1 and 2:1 (media:cell culture) ratio using L1 media. The two starter tubes were used (one was used for 2:1 ratio and the one for 5:1 ratio). Then cultures were incubated at 2.5 degrees C using a 16:8 (L:D) cycle. After seeing that the cultures were not performing well we also used some of the initial culture and transferred it in F2 media. So far the cultures do not look great although I hope that it might just be slow growing, and that hopefully by next week the cell numbers would have increased. I would like to hear if you have any suggestions on how to improve the growth of this strain.
A: Rhizosolenia setigera will tend to make auxospores, especially when it has been in the dark for a couple days. This culture is a slow grower, and with auxospore formation the regrow time may be a little longer. It may also be acclimating to a change in light cycle as well. It is cultured in a 13:11 L/D cycle.
Q:My colleagues are preparing a proposal which includes genome sequencing of a strain from your collection (CCMP1330). We wanted to check in with you to make sure that this is permitted by your policies, or whether we would have to complete a special MTA. If our proposal gets accepted, the genome results will be made publicly accessible.
A: No need for a special MTA. If you are successful in getting funding it would be great if you could send us links to the data in genbank or whereever so we can make that link on our end as well.