Isochrysis galbana  Parke

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$18.00 to $875.00
SKU: CCMP1323
Class: Prymnesiophyceae

Grown to Order

Common Namegolden algae
Collection Site54.085°N -4.77°W
Marine Biological Station, Port Erin, Isle of Man
OceanNorth Atlantic
SeaIrish Sea
Nearest ContinentEurope
Collected By
Collection Date
Isolated ByParke,M
Isolated Date01/01/1938
Identified ByParke,M
Deposited By
Deposit Date
Strain SynonymsISO;Plymouth I; UTEX 987; CCAP 927/1; NEPCC 2; NEPCC 633; SMBA 58;
Is The Strain Currently Axenic?Yes
When Was It Last Tested?09/07/2021
Other Informationsaltwater fish pond; EST sequence work done
Authentic Type/StrainYes
Morphological Data
AttributesAlgae, Marine, Robust, High Lipid, Temperate, Aquaculture
Additional ResourcesGenbank
Algaebase
Medium Used for MaintenanceL1 - Si
Other Reported Growth Mediaf/2-Si, L1 - Si
Maintenance Temperature (°C)14 °C
Known Temperature Range (°C)3 - 28 °C
Cell Length (Min)4
Cell Length (Max)6
Cell Width (Min)2
Cell Width (Max)4
CCMP1323 was cryopreserved on Dec 14 1999 using 10% DMSO as a cryoprotectant.

The time required to regrow this culture, prior shipping, is approximately 21 days. If interested, please contact the CCMP for the cryopreservation methods (freezing and/or thawing protocols).

Note that aquaculture strains are always maintained as actively growing cultures, even if also cryogenically stored. Therefore, aquaculture strains (see aquaculture express ordering on the CCMP home page) can be shipped immediately upon request.

Q:       We are having some trouble with our cultures and were wondering if you would be able to provide any additional advice or information. Some questions that we have or have run across as we began to grow the algae are as follows:

           *      Have you used instant ocean to prepare your medium?

           *      What colors we should be seeing and should we be seeing any sedimentation?

                   -  i.e. Is there a color change that would indicate the algae is unhealthy?   

           *      Do you have any references or pointers as to what timescale and methods are best for splitting and scaling-up the cultures (e.g. what density should we grow to before we split and what splitting ratio works best with I. galbana).

           *      Should we grow these in vented flasks or tubes or should closed glass tubes work?

           *      For larger scale production, do you recommend bubbling air or 1% CO2?

 A:       1.     We do not use instant ocean. Instant ocean will precipitate when autoclaved which may make key nutrients unavailable. 

           2.    CCMP1323 should be a nice light to golden brown when in a healthy culture. any other color such as green would indicate an unhealthy culture.

           3.     I like a 1 to 5 split for keeping a good growth rate and building biomass once a culture is healthy my co-worker prefers a 1 to 10 split. 1:1 is good if it is unhealthy. For this culture we put 1 drop into 18mls and it is grown in 3 weeks.

           4.     We use Kim caps on test tubes https://labgenome.com/73660-25-dwk-life-sciences-kimble-kim-kap-test-tube-caps-case-of-500/?gclid=EAIaIQobChMI7v2znd7b5wIViaztCh10ZgazEAQYASABEgLyyPD_BwE   or silicone stoppers in a flask. Open or vented will allow bacteria in. 

           5.     For volumes over 20L we sometimes use CO2.

           6.     I would not recommend agitation or shaking. 

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