Thalassiosira minuscula Schrader
| Common Name | centric diatom | ||||
| Collection Site | 32.9°N -117.255°W Scripps Institute of Oceanography, pier, La Jolla, California USA | ||||
| Ocean | North Pacific | ||||
| Sea | California Bight | ||||
| Nearest Continent | North America | ||||
| Collected By | Dodson,A | ||||
| Collection Date | |||||
| Isolated By | Dodson,A | ||||
| Isolated Date | 01/01/1970 | ||||
| Identified By | Alverson,A | ||||
| Deposited By | Thomas,W | ||||
| Deposit Date | 05/12/1982 | ||||
| Strain Synonyms | WTSIO | ||||
| Is The Strain Currently Axenic? | No | ||||
| When Was It Last Tested? | 09/18/1995 | ||||
| Other Information | |||||
| Authentic Type/Strain | No | ||||
| Morphological Data | |||||
| Attributes | Algae, Marine, Warm Water | ||||
| Additional Resources | Genbank Genome Transcriptome AlgaeBase | ||||
| Genome Sequence Link | No | ||||
| Medium Used for Maintenance | L1 |
| Other Reported Growth Media | f/2 |
| Maintenance Temperature (°C) | 24 °C |
| Known Temperature Range (°C) | 22 - 26 °C |
| Cell Length (Min) | 10 |
| Cell Length (Max) | 20 |
| Cell Width (Min) | 10 |
| Cell Width (Max) | 14 |
The time required to regrow this culture, prior shipping, is approximately 18 days. If interested, please contact the CCMP for the cryopreservation methods (freezing and/or thawing protocols).
Note that aquaculture strains are always maintained as actively growing cultures, even if also cryogenically stored. Therefore, aquaculture strains (see aquaculture express ordering on the CCMP home page) can be shipped immediately upon request.
- https://www.ncbi.nlm.nih.gov/nuccore/EF208799.1
- Q: Can you tell me what light and temperature conditions you keep the strains in? We are finding that we have to transfer the cultures every few days to keep them alive, which seems a bit strange - I can't imagine that you are continuously keeping a culture collection in exponential growth.
A: The strain is not in the perpetual collection. It is grown at ~100uE. The cryo pull is split 1:1 with fresh medium based on the time frame established by the cryo test run. A subset is created on the day of shipment to cover 2 weeks in case of shipment failure. All of our perpetual cultures are kept in borosilicate test tubes with 18mL of media. The transfer schedule we use is anywhere from 1 week to 10 weeks depending on the culture. The inoculum can be anywhere from 1 drop to 3mL, again depending on the culture. Every strain is kept in triplicate;grandmother, mother and daughter. We set the inoculum so that there are still viable cells in the grandmother, not to be used but they are there in case of a catastrophic incubator failure, by the time we reinoculate a daughter set.
Documentation:
Chitin in diatoms and its association with the cell wall (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2708456/)
Copper requirements for iron acquisition and growth of coastal ad oceanic diatoms (https://aslopubs.onlinelibrary.wiley.com/doi/abs/10.4319/lo.2005.50.4.1149)