Skeletonema grethae Zingone et Sarno
| Common Name | centric diatom | ||||
| Collection Site | 28.951°N -95.3642°W Freeport, Texas USA | ||||
| Ocean | North Atlantic | ||||
| Sea | Gulf of Mexico | ||||
| Nearest Continent | North America | ||||
| Collected By | Fryxell,G | ||||
| Collection Date | |||||
| Isolated By | Fryxell,G | ||||
| Isolated Date | |||||
| Identified By | Zingone, A & Sarno,D | ||||
| Deposited By | Fryxell,G | ||||
| Deposit Date | |||||
| Strain Synonyms | FRY2 | ||||
| Is The Strain Currently Axenic? | No | ||||
| When Was It Last Tested? | 10/25/2006 | ||||
| Other Information | |||||
| Authentic Type/Strain | No | ||||
| Morphological Data | chains | ||||
| Attributes | Algae, Marine, Temperate | ||||
| Additional Resources | Genbank Genome Transcriptome AlgaeBase | ||||
| Genome Sequence Link | No | ||||
| Medium Used for Maintenance | L1 |
| Other Reported Growth Media | f/2 |
| Maintenance Temperature (°C) | 14 °C |
| Known Temperature Range (°C) | 11 - 16 °C |
| Cell Length (Min) | 6 |
| Cell Length (Max) | 9 |
| Cell Width (Min) | 4 |
| Cell Width (Max) | 8 |
This lot is a result of a subculture made 19 May 2025 in L1 medium at 14º C. After removing about 10mL of overlying medium, the culture was mixed and dispensed into cryovials. They were exposed to cryoprotectant for 30 minutes before the CRF was started.
Three test vials were thawed at 20º C until there was just a little ice left. They were then spun at 7000 rpm for approx. 10 seconds in an eppendorf centrifuge to pellet the cells. The supernantant was removed and the cells resuspended and pipetted into14C tubes of L1. They were grown at 14º C in high light. The control was shippable after 10days. The thaws were shippable after 35 days. The bacto tests showed that the thaws were contaminated with bacteria as in the active collection. No fungal contamination was detected.
New daughter set created every four weeks
Daughter set……….…25umol quanta m-2 s-1
Mother set………….….13umol quanta m-2 s-1
Grandmother set….….7umol quanta m-2 s-1
Documentation:
Trait-dependent variability of the response of marine phytoplankton to oil and dispersant exposure (https://www.sciencedirect.com/science/article/pii/S0025326X20300242)
Physiological response of 10 phytoplankton species exposed to macondo oil and the dispersant, Corexit (https://pubmed.ncbi.nlm.nih.gov/29464721/)
Transport of dispersed oil compounds to the seafloor by sinking phytoplankton aggregates: A modeling study (https://www.sciencedirect.com/science/article/pii/S0967063719303383)