Nostoc_cf. sp.
| Common Name | blue-green algae | ||||
| Collection Site | 21.46°N -157.79°W Sampan Channel, Kaneohe Bay, Ohau, Hawaii USA | ||||
| Ocean | North Pacific | ||||
| Sea | |||||
| Nearest Continent | Micronesia | ||||
| Collected By | Messer,A | ||||
| Collection Date | |||||
| Isolated By | |||||
| Isolated Date | |||||
| Identified By | Andersen.R | ||||
| Deposited By | Tien,G | ||||
| Deposit Date | 10/19/2004 | ||||
| Strain Synonyms | CMMED01 | ||||
| Is The Strain Currently Axenic? | No | ||||
| When Was It Last Tested? | 02/04/2005 | ||||
| Other Information | 6 inches below the surface | ||||
| Authentic Type/Strain | No | ||||
| Morphological Data | filamentous | ||||
| Attributes | Algae, Toxic, Marine, Warm Water | ||||
| Additional Resources | Genbank Genome Transcriptome AlgaeBase | ||||
| Genome Sequence Link | No | ||||
| Medium Used for Maintenance | L1 - Si |
| Other Reported Growth Media | f/2-Si, L1 - Si |
| Maintenance Temperature (°C) | 24 °C |
| Known Temperature Range (°C) | 22 - 26 °C |
| Cell Length (Min) | 4 |
| Cell Length (Max) | 6 |
| Cell Width (Min) | 4 |
| Cell Width (Max) | 4 |
This culture was started in a tube of f/2-Si at 24 degrees C. Approximately 10mL of overlying medium was removed before mixing and dispensing the culture. The cells were exposed to the cryoprotectant for 42 minutes before the fullsw program was started. The probe was in the same final volume as the cells (1ml) and consisted of 5% DMSO diluted in f/2-Si.
Three test vials were thawed into room temperature 25ml tubes of f/2-Si medium . They were spun for 5 seconds at top speed in an eppendorf microfuge to remove the supernantant.They were grown in at 24 degrees in high light. In 12 days all three were shippable. The bact test showed that the thaws were contaminated with bacteria as in the active collection. No fungal contamination was detected.
Documentation:
Rapid quantitative screening of cyanobacteria for production of anatoxins using direct analysis in real time high-resolution mass spectrometry (https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/abs/10.1002/rcm.8940)