|Collection Site||46°N 104°W |
contaminated with a small green coccoid organism; saline lake, Mongolia (Cha-ga-nur), 90 km NE Wu-shen-chi China (lat long very approximate)
|Strain Synonyms||E-104, CCMP3490, JS1599|
|Is The Strain Currently Axenic?||No|
|When Was It Last Tested?|
|Other Information||pH 10, salinity = 230%. , CO3 = 14.48 g/L, K = 3.49 g/L, Cl = 29.4 g/L, Na = 31.93 g/L|
|Attributes||Algae, Marine, Hypersaline|
|Medium Used for Maintenance||L1 - Si|
|Other Reported Growth Media||f/2-Si, L1 - Si|
|Maintenance Temperature (°C)||20 °C|
|Known Temperature Range (°C)||18 - 22 °C|
|Cell Length (Min)||8|
|Cell Length (Max)||12|
|Cell Width (Min)||6|
|Cell Width (Max)||8|
The time required to regrow this culture, prior shipping, is approximately 32 days. If interested, please contact the CCMP for the cryopreservation methods (freezing and/or thawing protocols).
Note that aquaculture strains are always maintained as actively growing cultures, even if also cryogenically stored. Therefore, aquaculture strains (see aquaculture express ordering on the CCMP home page) can be shipped immediately upon request.
Q: We would appreciate if you could please share the protocol you used for the cryopreservation of this culture so that we do not attempt that and try other approaches.
A: This is what we have in the database for CCMP189 cryo event. This culture was started in a tube of L1+/ 50uM NH4Cl/ 0.075% agar medium at 4 degrees C. The culture was mixed and dispensed into cryovials. They were exposed to cold cryoprotectant for 34 minutes before the full sw program was started. The probe was in a final volume of 1ml and consisted of 5% DMSO diluted in f/2-Si. Three test vials were also frozen. Everything was cold. Three test vials were thawed at 37 degrees just until the ice started to melt and the entire contents of the cryovial could be dumped into ice cold L1+. The thaws were then grown at 4 degrees in box5. The control was shippable after 32 days but the thaws never grew.